Coding

Part:BBa_K5005008:Design

Designed by: YunQi Chen   Group: iGEM23_GEC-CHINA   (2023-10-05)


The product of NAMPT point mutation - D354E.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 879
    Illegal BglII site found at 1176
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1343


Design Notes

To verify the point mutation in the patent CN 108026517 B,caaggggaaggagtagatattaataccttacaagag is the forward primer of eN-D354E-F. gtaaggtattaatatctactccttccccttgaataactc is the reverse primer of eN-D354E-R.These two primers are used for point mutation of NMAPT which helps NAD+ systhesis.


Source

Constructed in this work.

References

Zhu, X., Liu, H., Chen, L., Wu, C., Liu, X., Cang, Y., Jiang, B., Yang, X., & Fan, G. (2022). Addressing the Enzyme-independent tumor-promoting function of NAMPT via PROTAC-mediated degradation. Cell chemical biology, 29(11), 1616–1629.e12. https://doi.org/10.1016/j.chembiol.2022.10.007